Effect of puerarin on glucogen synthase Kinase-3 bate in adipocyte of rats with insulin resistance / 中国临床药理学与治疗学

ABSTRACT

AIM: To investigate the effect of puerarin on expression of the glucogen synthase kinase-3 bate (GSK-3?) in adipocyte of rats with insulin resistance induced by diets. METHODS: 30 Wister rats were randomized into two groups control group (n=10) and model group (n=20).Control group were fed with general diets, while model group were fed with the high fat diets to induce insulin resistance. After 4 weeks, the models have formed then were randomized into two groups model group (n=10) and puerarin group (n=10). Puerarin group were treated with puerarin (100 mg?kg -1 ?d -1 , ip) for 6 weeks.GSK-3? protein was detected by Western-blot analysis. Body weight, serum triglyceride and cholesterol, fasting plasma glucose and serum insulin concentration were detected termly and the insulin sensitivity index was calculated. RESULTS: The model group showed hyperglycemia, hyperinsulinism, obviously obesity and the insulin resistance index (ISI) decrease by high fat diets, insulin resistance was induced by this way. Compared with model group, GSK-3? protein in adipocyte of puerarin group rats decreased (P