Comparing the characteristics of rat pulmonary arterial smooth muscle cell cultured by two methods / 第三军医大学学报

ABSTRACT

Objective To compare the growth curve, protein content, calcium ion content of pulmonary arterial smooth muscle cells (PASMCs) cultured by enzyme digestion and tissue-sticking methods. To investigate whether PASMCs cultured by two ways could be offered to one series of signaling pathway studies. Methods The protein content was determined by upgraded-lowry, the calcium ion content by fluorescence indicator. The growth of PASMCs was observed by light microscope. Results There existed certain difference in protein content, growth curve between PASMCs cultured by the two methods, especially in calcium ion content (P