Inhibition of xathine oxidase by renierol extracted from South China Sea sponge / 第二军医大学学报

ABSTRACT

Objective:To study the inhibition of xanthine oxidase by renierol extracted from South China Sea sponge and study the influence of renierol on hyperuricemia in mice.Methods: After extracted from South China Sea sponge,renierol(20,40,60 ?g/ml) was added to a system containing xanthine oxidase(0.1 ?/ml) and xanthine(50 ?mol/ml);allopurinol((1 ?g/ml))was also addded to the system as positive control.The 5 min-fomation of superoxide anions was used to determine the activity of xanthine oxidase(Nitro Blue Btetrazolium reduction).Renierol(20,40,60 ?g/ml) was added to 25℃ pre-heated pyrogallol autoxidation to observe its eliminating effect on free radicals through determining the absorbance rate at 420 nm wavelength.Potassium oxonate,a uricase inhibitor,was used to induce hyperuricemia in mice and the mice were then treated with oral renierol(10,20,30 mg?kg~(-1)) or allopurinol(2 mg/kg) as positive control.The decrease of serum uric acid induced by renierol was determined by automatic biochemical analyzer.Results: Renierol was demonstrated to be a competitive inhibitor of xanthine oxidase in vitro,with its IC_(50) value being 1.36 ?g?ml~(-1).It also decreased uric acid in vivo.Conclusion: Renierol can decrease serum uric acid through inhibiting the xanthine oxidase.