The effects of catalase on expression of cytokines and activation of nuclear factor ?B in intestinal mucosa in rat with ulcerative colitis / 解放军医学杂志

ABSTRACT

Objective To investigate the effects of catalase on expression of cytokines and activation of nuclear factor-?B in the intestinal mucosa in rat with ulcerative colitis(UC). Methods UC was reproduced in rats with the oral innoculation of dextran sulfate sodium (DSS).The expressions of TNF-?, IL-1? and IL-8 in the intestinal mucosa of rats were respectively determined by a semi-quantatitive assay, RT-PCR. The activation of NF-?B in the intestinal mucosa were assessed with electrophoretic mobility shift assay (EMSA). Results Comparing the mice challenged with DSS alone and to those treated with catalase, both symptoms and the lesions in the colonic mucosa were milder in the animals pretreated with catalase during the induction of colitis than that of control group and catalase treatment after induction of colitis group. Furthermore, the expressions of TNF-?, IL-1?, IL-8 were significantly down-regulated(P