Triplex forming oligonucleotide of PDGF-B chain combined with antisense oligonucleotide of VEGF inhibits glioma growth in rats / 第二军医大学学报

ABSTRACT

Objective:To observe the inhibitory effect of triplex forming oligonucleotede(TFO) of PDGF-B chain combined with antisense oligonucleotide(AON) of VEGF on glioma growth in rats.Methods: Thirty-six male SD rats received 1?10~(6) C_(6) glioma cells/20 ?l normal saline into the right caudate putamen by stereotactic technique.GroupⅠ(n=6) was treated in situ with 1.5 mg/20 ?l TFO for 3 times on 8,11 and 14 d after glioma cell inoculation.Group Ⅱ(n=12) and Ⅲ(n=12) were respectively treated with TFO 1.5 mg+AON 0.125 mg/20 ?l and TFO 1.5 mg+AON 0.250 mg/20 ?l for 3 times on 8,11 and 14 d after glioma cell inoculation.Control group(n=6) was treated with 20 ?l saline for 3 times at the same time pionts.Three weeks after cell inoculation,all rats were sacrificed to observe tumor growth and to determine the expression of PDGF-B,VEGF and PCNA.Results: The inhibition rate of tumor growth was 53.1% in groupⅠ,81.4% in group Ⅱ and 93.1% in group Ⅲ,with significant difference found between the 3 groups(P