Effects of mycophenolic acid on proliferation and collagen I of unstimulated or stimulated vascular smooth muscle cells / 中国实用内科杂志

ABSTRACT

Objective To develop stable cultures of human umbilical cord vascular endothelial cells(ECs)and smooth muscle cells(SMCs)in order to investigate the effect of mycophenolic acid(MPA)on proliferation and collagen Ⅰ production of SMCs.Methods From Sep.2002 to Sep.2003,ECs were cultured from human umbilical cord veins,and SMCs from arteries.Productions of endothelial cells conditioned medium(ECCM)was obtained from serum free Dulbecco's modified eagle medium(DMEM)with or without MPA(0,0.31,1.25,2.50,5.00,10.00 ?g?mL -1).Proliferation of SMCs was performed with 3H-thymidine incorporation scintillation,and collagen Ⅰ production of SMCs was measured with enzyme-linked immunosorbent assay.Results (1)The 3H-thymidine incorporation increased significantly(P