Changes of XOD activity induced by acute urinary retention in rats / 第三军医大学学报

ABSTRACT

Objective To study the changes of xanthine oxidoreductase(XOD) activity induced by acute urinary retention(AUR) in rats and its significance.Methods Twenty-eight female Wistar rats were used to establish acute urinary retention model by filling bladder with 2fold bladder volume and maintaining for 2 h.Cystometry,XOD activity and malondialdehyde(MDA) concentration assays were performed at the end of over-distention and 1 h,2 h,3 h,4 h after emptying respectively.The effect of superoxide dismutase(SOD) was observed by injecting 500 U SOD into the left cardiac ventricle of 7 model rats 10 min before emptying.Another 7 rats were filled with normal bladder volume as controls and underwent filling cystonetry.Results The frequency of detrusor over-activity increased more obviously after emptying than during over-distention.XOD activity increased obviously during over-distention period as compared to controls,reduced 1 h after emptying,then increased again 2 h after emptying,finally down to normal level slowly.The concentration of MDA increased during over-distention and early period after emptying and peaked at 1 h after emptying and then recovered slowly.After SOD treatment,the frequency of detrusor over-activity,XOD activity and MDA concentration reduced as compared with that in model rats 2 h after emptying(P