Effects of ketamine on expression of nitric oxide synthase in medial septal nuclei and vertical diagonal band of rats / 中国临床药理学与治疗学

ABSTRACT

AIM: To explore the effects in different dosage of ketamine on the expression of nitric oxide synthase (NOS) in medial septal nuclei (MS) and vertical diagonal band (VDB) of rats. METHODS: 40 SD rats were randomized into normal control group,normal saline(NS) group, low, middle and high dosage of ketamine(25,50,100 mg?kg~ -1 ,ip) group.Each group was maintained intraperitoneally by given daily dosage once a day for a period up to 7 days. That was done at 2100 hour each night, and by 2 h of the last administration, poured, fixed and sliced were carried out. Method of nicotinamide adenosine dinucleotide phosphate diaphorase (NADPH-d) was used to show the expression of NOS in MS and VDB pars dorsalis and ventro. RESULTS: Compared with normal control and NS group, the number of NOS positive neurons were decreased in MS and VDB pars dorsalis and ventro in ketamine groups.There were statistical meaing in different-dosage groups of ketamine(P