Analysis of the mitochondrial mutations of articular chondrocyte in temporomandibular joint osteoarthrosis / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6)2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-559184
ABSTRACT
Objective:
To explore the significance of mitochondrial DNA mutations of articular chondrocytes in the rat model of temporomandibular joint osteoarthrosis (TMJOA).Methods:
TMJOA models were created in left sides of TMJ of 15 SD rats by the partial resection of the articular disc.The experimental rats were killed 3 months after operation. After the chondrocytes culture, the entire mtDNA were amplified using 32 pairs of overlapping. DNA fragments showing different banding patterns between normal and experimental mtDNA by temporal temperature gradient gel electrophoresis were sequenced to identify the mutations.Results:
Of the 35 heteroplasmic pattern PCR products, 42 novel mutations were found, Majority of the novel mutations were in the tRNA and D-loop regions.Conclusion:
The mutations occurred in the mtDNA of TMJOA articular chondrocytes.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Peking University(Health Sciences)
Ano de publicação:
2004
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS