Effect of PKC? inhibitor on permeability injury of pulmonary microvascular endothelial cells monolayer induced by lipopolysaccharide / 中国药理学通报

ABSTRACT

Aim To study the expression of protein kinase C(PKC) subtype PKC? in rat pulmonary microvascular endothelial cell(RPMVEC),and the effect of its inhibitor(Rottlerin) on LPS-induced RPMVEC monolayer permeability injury.Methods In cultured RPMVEC,Western blot and immunohistochemistry were used to identify the expression of PKC?.Micro-infiltrator was used to measure the changes of RPMVEC monolayer permeability coefficient(Kf) after exposure to LPS or LPS with Rotterin together.Result We found a high level of PKC? expression in cytoplasm.Rottlerin could obviously inhibit the increase of LPS-induced RPMVEC Kf value.Conclusion The activation of PKC is involved in the progress of LPS-induced RPMVEC injury.Furthermore,the inhibitor of PKC? could release LPS-induced RPMVEC Kf increase.