Inhibitory effect of Epibueropyridium A extracted from scrophularia ningpoensis on aldose reductase / 第二军医大学学报

ABSTRACT

Objective:To investigate the inhibitory effect of Epibueropyridinium A on aldose reductase.Methods: Aldose reductase was extracted from cattle crystalline lens.Besides Epibueropyridinium A,the reactive system also contained DL-glyceraldehyde,aldose reductase,and NADPH.The activity changes of aldose reductase were detected at 340 nm.Epalrestat was taken as the positive control.The inhibitory type,Ki and IC_(50) were determined by double reciprocal plot,quadratic drawing,and drawing of inhibitor's concentration to inhibitory ratio,respectively.Results: Epibueropyridinium A significantly inhibited the activity of aldose reductase in a competitive manner,with IC_(50) being 4.2 ?g/ml and Ki being 4.88 ?g/ml.Conclusion: Epibueropyridinium A is competitive inhibitor of aldose reductase.