Expression of recombined human immunoglobulin ? light chain 022 and TOM1 mRNA in omental adipose tissues of patients with type 2 diabetes and insulin resistance / 第二军医大学学报

ABSTRACT

Objective:To investigate the expression of recombined human immunoglobulin ? light chain 022 and TOM1 gene and to determine the protein expression of HSIGVL022 in omental adipose tissues of patients with type 2 diabetes and insulin resistance(T2DM-IR).Methods: Fat tissues from greater omentum of T2DM-IR patients and normal controls were obtained.The mRNA levels of recombined human immunoglobulin ? light chain 022 and TOM1 were measured by fluorescent quantitative RT-PCR;expression of HSIGVL022 protein was identified by immunohistochemistry.Results: The mRNA levels of HSIGVL022 and TOM1 in T2DM-IR patients were(34 140?6 160) copy/million house-keeping genes and(4 440?617) copy/million house-keeping genes,respectively;those in control group were(5 930?661) copy/million house-keeping genes and(1 360?82) copy/million house-keeping genes,respectively.There were significant difference between the 2 groups(P