Protective effect of puerarin on anoxia-reoxygenation induced cerebral cell injury in culture / 中国药理学通报
Chinese Pharmacological Bulletin
;
(12)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-559933
ABSTRACT
Aim To study the protective effect of puerarin(PUE) on cultured cerebral cells injured by anoxia-reoxygenation. Methods The anoxia-reoxygenation injury model were developed, anoxia for 60 min and reoxygenation for 30 min. the effect of PUE on cerebral ultrastructure was observed.[Ca 2+ ]_i was estimated with Adherent Cell Analysis and Sorting 570(ACAS 570) Laser Cytometer and measured with fluorescent dye Fura-2-AM, the lipid fluidity of cellular membrane was determined by fluorescence polarization technique. Results PUE improved the ultra-structure of cerebral cells, dose-dependently decreased[Ca 2+ ]_i and increased the lipid fluidity of cellular membrane. PUE markedly reduced the chromaticity value of pseudo-colour graphic model of Ca 2+ . Conclusion Puerarin has protective effect on anoxia-reoxygenation induced cerebral cell injury. The effect may be mediated by decreasing[Ca 2+ ]_i and increasing the lipid fluidity of cellular membrane.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2003
Tipo de documento:
Artigo
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