Preliminary studies on the expression of bivalent DNA vaccine against Schistosoma japonicum in vitro/vivo and lasting time / 中国血吸虫病防治杂志

ABSTRACT

Objective To study the expression of bivalent DNA vaccine against Schistosoma japonicum in vitro/vivo and lasting time in vivo. Methods The bivalent DNA pVIVO2-SjFABP/Sj23 was transfected transiently into the human embryonic kidney cells of the line 293. RT-PCR was used to detect the expressions of SjFABP and Sj23 mRNA in the 293 cells. Indirect immunofluorescence antibody test(IFAT) was used to detect the expressions of SjFABP and Sj23 proteins. Twelve BALB/c mice were inoculated with pVIVO2-SjFABP/Sj23 via intramuscular injection. Two blood samples were collected from eye sockets and two mice were vivi-perfused each month. This work lasted for 6 months. IFAT was used to detect the expression of proteins in skeletal muscle. Western-blot was used to detect the serum IgG level. Results RT-PCR and IFAT showed the expressions of SjFABP and Sj23 in the HEK-293 cells. IFAT showed SjFABP/ Sj23 was present in vivo at least for 6 months. Special IgG was not detected by Western blot. Conclusions pVIVO2-SjFABP/Sj23, bivalent DNA vaccine, could express correctly in vitro/vivo, and lasts for 6 months at least in vivo.