STUDY ON THE EFFECT OF IODINE EXCESS ON CATHEPSINS IN RAT THYROID CELLS / 营养学报
Acta Nutrimenta Sinica
;
(6)2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-560256
ABSTRACT
Objective:
To explore the effect of iodine overdose on cathepsin B and D in thyroidcell line (FRTL).Method:
The cells were treated with potassium iodide (0, 1, 5, 10, 50 and 100mmol/L). After treatment 6, 12, 24, 48 and 72 hours, the cells were collected. The activities of cathepsin B (CB) and cathepsin D (CD) were assayed by fluorospectrophotometry and Lowry, respectively. The mRNA levels of CB and CD were determined with real-time PCR.Results:
After 12 and 24 hours, the activities of CB and CD, treated by 50mmol/L potassium iodide were decreased significanty. The mRNA levels of CB and CD of 50mmol/L potassium iodide group were decreased significanty after treatment 24 hours.Conclusion:
The decreased activities of CB and CD were one of reasons of thyroid colloid retention resulted from iodine excess.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Acta Nutrimenta Sinica
Ano de publicação:
2004
Tipo de documento:
Artigo
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