Change in ghrelin level with the amelioration of glucose and lipid metabolic disorder in OLETF rats / 北京大学学报(医学版)

ABSTRACT

Objective: To explore the exact role of ghrelin in glyco-and lipo-metabolism.Methods: We compared the levels of ghrelin mRNA in gastric tissue,ghrelin in gastric tissue and plasma among LETO rats(non diabetes,n=10),OLETF rats(type 2 diabetes,n=10),OLETF/M rats(OLETF rats managed with Metformin at the dose of 100 mg/kg weight,n=10) and OLETF/F rats(OLETF rats managed with Fenofibrate at the dose of 20 mg/kg weight,n=10).The levels of ghrelin mRNA were tested by Northern blotting,and the ghrelin content in gastric tissue and plasma detected by RIA.Results: At the age of 30 weeks,the ghrelin fasting plasma levels of OLETF rats were lower than those of LETO rats(37.49?6.42 vs 58.52?5.85,P0.05).However,the fasting blood plasma ghrelin levels of OLETF/F group were more than those that of the untreated OLETF rats(62.02?7.35 vs 37.49?6.42,P