In vivo transfer of human chemokine-like factor 1 gene increases peripheral blood CD34~+ stem cells after myocardial infarction in rats / 北京大学学报(医学版)

ABSTRACT

Objective: To assess the influence of different doses of CKLF1 plasmid on the dynamics and magnitude of the mobilization of the mobilization bone of marrow stem cells in a rat AMI model.Methods: Different doses of plasmid DNA encoding CKLF1 gene,empty plasmid or saline were injected into male SD rats intramuscularly with in vivo electroporation.Rats were subjected to left coronary artery ligation 6 days after gene transfer.Peripheral blood samples were drawn and CD34~+ cells were assayed by FACS calibur flow-cytometer.The changes in absolute number of CD34~+ cells were evaluated.Results: Expressions of CKLF1 mRNA and protein were detected in the injection site 7 days after gene transfer.Five days after gene transfer,the CD34~+ cells numbers in CKLF1 groups were significantly higher than those in empty plasmid group,especially in CKLF1 100?g group(16.63?10~6/L vs 4.98?10~6/L,P