INHIBITION OF OXIDIZED LIPOPROTEINS INDUCED APOPTOSIS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS BY TEA POLYPHENOLS / 营养学报
Acta Nutrimenta Sinica
; (6)2004.
Article
em Zh
| WPRIM
| ID: wpr-560695
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WPRO
ABSTRACT
Objective: To study the inhibition and mechanism of tea polyphenols (TP) on apoptosis induced by oxidized low density lipoprotein (ox-LDL) in human umbilical vein endothelial cells (HUVEC). Method: HUVEC cells were administered with TP (25 ?g/ml), ox-LDL (200 ?g/ml)+TP (25 ?g/ml), ox-LDL (200 ?g/ml) respectively, cells with equal volume of solvent as control. Cell viability was determined by MTT assay, apoptosis by acridine orange fluorescence dyeing, and expressions of Bcl-2、Bax and caspase-3 were analyzed by Western blotting. Results: Ox-LDL could inhibit HUVEC cell proliferation. After treated with both TP and ox-LDL , the cell proliferation increased obviously and showed significant difference from the cells treated with ox-LDL only (P
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Idioma:
Zh
Revista:
Acta Nutrimenta Sinica
Ano de publicação:
2004
Tipo de documento:
Article