Cytokine release in co-culture of eosinophils and BEAS-2B cells is regulated by p38 MAPK / 解放军医学杂志

ABSTRACT

Objective To investigate p38 MAPK signal transduction pathway of cytokine release in co-culture of BEAS-2B cells and eosinophils.Methods Eosinophils in human peripheral blood were isolated using anti-CD16 magnetic micro beads.Co-culture of BEAS-2B cells and eosinophils was taken as experimental model to investigate the inhibitive effect of SB 203580,a selective inhibitor of p38 MAPK,on release of IL-6,IL-8,IP-10 and MIG in culture supernatant.IL-6 was determined by ELISA kit and IL-8,IP-10 and MIG were measured using cytometric bead array(CBA)kit.Results SB 203580 could inhibit IL-6 and IL-8 release from BEAS-2B cells(P