Prevention of Vasculogenic Erectile Dysfunction in Rat Model by the Chronic Administration of Oral Rho Kinase Inhibitor / 대한비뇨기과학회지
Korean Journal of Urology
;
: 322-330, 2006.
Artigo
em Coreano
| WPRIM
| ID: wpr-56094
ABSTRACT
PURPOSE:
Since Rho-Rho kinase calcium sensitizing pathway is being regarded as a potential target not only for the treatment of erectile dysfunction but also atherosclerosis, we designed a study to prevent vasculogenic erectile dysfunction in an atherosclerotic rat model by chronic administration of fasudil, oral Rho kinase inhibitor. MATERIALS ANDMETHODS:
Rats (3 months old) were divided into 3 groups (n=10 in each group) control (group 1), atherosclerosis (group 2), and fasudil-treated (group 3). The group 2, 3 received atherosclerosis-prone treatment but group 3 was concurrently treated by fasudil (30mg/kg/day) for 6 weeks. Following the treatment, the erectile function and the amount of pelvic atherosclerosis amount were determined. Cavernosal tissues were prepared for Western blot and malondialdehyde (MDA) assay.RESULTS:
Compared to group 2, the progression of atherosclerosis in iliac and pudendal arteries was significantly suppressed by the chronic administration of fasudil. Also the treatment lowered the level of malondialdehyde in the cavernosal tissue. The results of Western blot revealed that systemically administered fasudil could ameliorate cavernosal molecular environment characterized by the decreased expression of Rho A, transforming growth factor-beta 1 and overexpression of endothelial nitric oxide synthase. As a result, erectile function was maintained by the treatment.CONCLUSIONS:
These results indicate that Rho/Rho kinase pathway is substantially involved in the development of penile erection and pelvic atherosclerosis, both of which could be prevented by chronic treatment of fasudil. Thus, Rho-kinase might be considered as a novel target for prevention of vasculogenic erectile dysfunction.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Artérias
/
Ereção Peniana
/
Western Blotting
/
Cálcio
/
Impotência Vasculogênica
/
Proteínas rho de Ligação ao GTP
/
Modelos Animais
/
Aterosclerose
/
Óxido Nítrico Sintase Tipo III
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Coreano
Revista:
Korean Journal of Urology
Ano de publicação:
2006
Tipo de documento:
Artigo
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