REGULATION OF EXOGENOUS POLYAMINES ON ORNITHINE DECARBOXYLASE GENE TRANSCRIPTION IN REGENERATING RAT HEPATOCYTES / 解剖学报

ABSTRACT

Objective To study the regulation of natural polyamines(putrescine,spermidine and spermine) on ornithine decarboxylase gene transcription in regenerating rat hepatocytes and to assess the roles of polyamines in liver regeneration. Methods Rat regenerating liver was induced by surgical partial hepatectomy(PH).The expressive levels of ODC mRNA in regenerating liver were measured by in situ hybridization analysis.Exogenous polyamines(dissolved in 0.9% NaCl) were administered subcutaneously to adult male Sprague-Dawley rats. Results ODC mRNA expression was always lower in high dose(20 mg/kg body weight) putrescine-treated rats compared with that in control rats during the observed regeneration,especially at the 2(nd),4(th) and 10(th) hours(P