Inhibition effect on LPS-induced TNF-? release by homegrown and Australian Melaleuca alternifolia(Tea Tree)oil / 第三军医大学学报

ABSTRACT

Objective To compare the inhibition of the homegrown and Australian tea tree oil(TTO)solution on TNF-? release from RAW264.7 cells induced by LPS.Methods MTT assay was used to measure the effects of TTO on viability of RAW264.7 in vitro.RAW264.7 cells were pretreated with two kinds of TTO solution in the dose-and time-dependent manners,then stimulated by LPS.TNF-? in the supernate was tested by ELISA.Results In vitro,two kinds of TTO(