Comparative proteomic analysis of differently differentiated human laryngeal squamous cell cancer / 第三军医大学学报
Journal of Third Military Medical University
; (24)1988.
Article
em Zh
| WPRIM
| ID: wpr-561184
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish two-dimensional electrophoresis profiles of laryngeal squamous carcinoma tissue with high resolution and reproducibility. Methods The total protein of well-differentiated or poorly-differentiated laryngeal cancer tissue was separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis (2-DE). The differential expression protein was analyzed by using ImageMaster 2D analysis software. Results We obtained fine resolution, reproducible 2-DE patterns of well-differentiated and poorly-differentiated laryngeal squamous carcinoma. Differential expression protein spots were defined in 2-DE gels. Thirteen protein spots were preliminarily identified, among which seven proteins were upregulated in well-differentiated laryngeal cancer tissue, six were downregulated. Conclusion The expression map of proteomics was established for well-differentiated and poorly-differentiated laryngeal cancer, which was of significant difference between them. Laryngeal cancer proteome might reflect the underlying pathological state.
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WPRIM
Idioma:
Zh
Revista:
Journal of Third Military Medical University
Ano de publicação:
1988
Tipo de documento:
Article