Prokaryotic expression and identification of human HT036 protein / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-561474
ABSTRACT
Objective To express human HT036 protein in Escherichia coli(E.coli.)and identify it.Methods The cDNA sequence obtained by PCR was cloned into the prokaryotic expression plasmid pET30a(+).The target protein was expressed in E.coli..induced by IPTG and analyzed by Western blotting.Results The interest gene was identified by restriction endonucleases digestion and DNA sequencing.The protein was highly expressed in E.coli..Conclusion We successfully expressed the HT036 protein.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Third Military Medical University
Ano de publicação:
2003
Tipo de documento:
Artigo
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