Study on Whole Blood Red Cell Lysing Reagent for Flow Cytometry / 医学研究杂志

ABSTRACT

Objective To study and develop the whole blood red cell lysing reagents in order to replace the commercial kit and reduce the cost.Methods Flow cytometry was used to compare the home-made red cell lysing reagents with the commercial kit on the separation of white cells into three groups,on the ratio of lymphocyte subsets and on the mean fluorescence intensity(MFI)of lymphocyte subsets.Results Compared with the commercial kit,the home-made lysing reagents had no significant difference on the separation of white cells into three groups,on the ratio of lymphocyte subsets and on the MFI of T lymphocytes,B lymphocytes,Helper-Inducer T-lymphocytes and Suppressor-Cytotoxic T-lymphocytes.Conclusions The home-made lysing reagents had similar effects as the commercial kit on the separation of white cells,on the ratio of lymphocyte subsets and on the MFI of T lymphocytes,B lymphocytes,Helper-Inducer T-Lymphocytes and Suppressor-Cytotoxic T-lymphocytes,but the cost is much lower.