Down-regulation effect of glucosides of cheanomeles speciosa on the levels of serum antibodies in rats with adjuvant arthritis / 中国药理学通报

ABSTRACT

Aim To study the effects of glucosides of cheanomeles speciosa(GCS)on the levels of serum antibodies in rats with adjuvant arthritis.Methods SD rats were divided randomly into six groups, including normal, model, GCS(30,60,120 mg?kg-1)and glucosides of tripterygium wifordii(GTW) groups. Complete Freund′s adjuvant was used to induce AA in rats. The level of circulating immune complexes in serum was determined by PEG 6 000 assay, while the levels of anti-CⅡ antibody, and anti-TB antibody in serum were measured by enzyme-linked immunosorbent assay(ELISA).Results GCS(30,60,120 mg?kg-1)and GTW(40 mg?kg-1)were given by intragastric administration for 8 days from the 17 th day after immunization. GCS(60,120 mg?kg-1)reduced the levels of circulating immune complexes, anti-CⅡantibody, and anti-TB antibody in serum in rats with adjuvant arthritis.Conclusions GCS down regulates the levels of serum antibodies in rats with adjuvant arthritis, which may be related to its efficacy in the treatment of rats with AA.