Effects of zinc finger protein A20 on lipopolysaccharide responses in human monocytes / 第三军医大学学报

ABSTRACT

Objective To investigate the effect of zinc finger protein A20 expression on lipopolysaccharide(LPS)response in human monocytes so as to discuss its regulation roles and mechanism to inflammation in vivo and its potential value of clinical application.Methods Cell transfection technique was respectively used to make zinc finger protein A20 transgenic cell model and gene silence cell model,both of which were then treated with LPS.Real-time reverse PCR was employed to analyze the expression of zinc finger protein A20 and ELISA to determine the activity of nuclear transcription factor NF-?B and the levels of dependent inflammatory cytokines including tumor necrosis factor(TNF)alpha.Results After LPS treatment,the NF-?B activity and the TNF-alpha level were significantly lower in the cells with overexpressed zinc finger protein A20 than LPS control cells(P