IDENTIFICATION AND TISSUE EXPRESSION OF NOVEL GENES IN DIABETIC RATS SUPPLEMENTED WITH SELENIUM / 营养学报

ABSTRACT

Objective: To clone novel gene fragments differentially expressed from diabetic rats supplemented with selenium and detect their expression distribution in various tissues. Method: cDNA fragments from former research project were cloned, sequenced and BLASTn analysed. The RT-PCR of the five novel genes were made using the primers designed according to the sequence of cDNA to observe the expression changes in liver of various groups and their expression distributions in various tissues. Results: Se-2, Se-6, Se-10, Se-14 and Se-18 cDNA were shown to be the novel gene fragments for no matched gene with them in GenBank. The expression levels of four cDNAs, including Se-2, Se-10, Se-14, Se-18, in DM group and DM+Se group were obviously lower than those in NC group. The expression level of DM+Se group was higher than those in DM group (P