Establishment of a "Four-step method" for extraction and separation of Candida albicans total proteins / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-562153
ABSTRACT
Objective:
To introduce a "Four-step method" for extraction and separation of Candida albicans total proteins.Methods:
Proteins of C.albicans were extracted step-by-step with 4 kinds of solutions with different solubilities.After quantification,the protein samples were separated by isoelectric focusing electrophoresis and then by SDS-PAGE.Results:
Proteins with different solubilities were successfully extracted step-by-step from C.albicans and were separated by two-dimensional gel electrophoresis.Conclusion:
The "Four-step method" for extraction of C.albicans proteins is an effective approach to study C.albicans membrane proteome and lays a foundation for further investigation of the mechanisms of antifungal agents and drug resistance in C.albicans.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2001
Tipo de documento:
Artigo
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