STUDY ON EFFECT OF RESISTANT STARCH ON EXPRESSION OF GENES ASSOCIATED WITH CHOLESTEROL METABOLISM IN RAT LIVER / 营养学报

ABSTRACT

Objective: To examine the effects of RS on serum cholesterol and expression of genes associated with cholesterol metabolism in rat liver for understanding its mechanism. Method: Eighteen rats were randomly assigned to three groups, six rats each. They were fed with chemical purified normal diet (control group), low RS (15%Hi-maize 1043) diet and high RS (30%Hi-maize 1043) diet respectively. Six weeks later, serum cholesterol and short chain fatty acid (SCFA) in the cecum were measured. Likewise, hepatic cholesterol 7?-hydroxylase, LDL receptor, and SR-B1 mRNA levels were measured by realtime PCR. Results: Hepatic cholesterol 7a-hydroxylase, LDL receptor, SR-B1 mRNA levels and cecal acetate, propionate and n-butyrate concentrations in the high RS group were significantly higher than those in the control group (P