Effect of transcription factor AP-2? on expression of tyrosine hydroxylase in locus ceruleus after traumatic brain injury / 第三军医大学学报

ABSTRACT

Objective To explore the role of transcription factor activator protein 2?(AP-2?) on the expression of tyrosine hydroxylase(TH) in noradrenergic neurons of locus ceruleus(LC) after traumatic brain injury.Methods Oinety rats were!randomly divided into control group and injury group that comprised 5 subgroups.There were 15 rats in each group.The rat model of traumatic brain injury was established with Feeney's methods.Brain stems were dissected from decapitated heads 1,3,6,24 and 72 h after traumatic injury and freeze-mounted for cryo-sectioning.TH and AP-2? expressions in noradrenergic neurons of LC were analyzed with double immunofluorescence.Results The number and fluorescence intensity of TH and AP-2?-positive neurons in LC at various intervals after injury significantly increased as compared with control group(P