Preparation and Application of Polyclonal Antibody Induced by SAK and Its Mutant / 医学研究杂志

ABSTRACT

Objective To study on the immunogenicity of SAK and its mutant using PAb.Methods 4 New Zealand white rabbits subcutaneously injected with corresponding antigens were grouped into group SAK involving 2 rabbits and group SAK2 involving 2 rabbits.The antiserums were collected 1 week after third injection.The rabbit IgG fraction was precipitated with saturated(NH4)2SO4 and purified by DEAE-Sepharose column chromatography,and then the antibody titer was determined by ELISA.The immunoreactivity of antigen to polyclonal antibody against SAK and SAK2 was tested using ELISA.Results The immunoreactivity of SAK2 to polyclonal antibody against SAK was sharply reduced to a very low level determined by ELISA,which indicated that some epitopes of SAK had been deleted.Conclusions The immunoreactivity of SAK to polyclonal antibody against SAK2 did not get a dramatically change compared with SAK2,which indicated that the reconstruction of epitope did not create new epitope.