Effect of Tang Ke Decoction on TNF-? and GLUT4 expression in tissues of insulin resistance of type two diabetes mellitus rats / 中华中医药杂志

ABSTRACT

Objective: To study the effect of Tang Ke Decoction on tumor necrosis factor ?(TNF?) and glucose transporter 4(GLUT4) expression in tissues of model rats of insulin resistance of type two diabetes mellitus,and to investigate the mechanism of the function of Tang Ke Decoction to insulin resistance.Methods: Rat model of insulin resistance of type two diabetes mellitus was duplicated by using strepozotocin(STZ) and high caloric diet,and using Tang Ke Decoction of compound Chinese herbs and rosiglitazone acted on model rats individually.The degree of insulin resistance was measured by insulin sensitivity index(ISI).The expression of TNF? mRNA was assayed in adipose tissue by RT-PCR.GLUT4 protein expression was assayed by immunohistochemical method in skeletal muscule tissue,and its distribution in skeletal muscle cell was observed with electron microscope.Results: ISI of model group was significantly lower than that of control group(P