Function study on cryopreservation of APA microencapsulated bovine chromaffin cells / 国际生物医学工程杂志

ABSTRACT

Objective To observe the morphology, cell viability and secretion function of catecholamine of the alginate-polylysine-alginate (APA) microencapsulated bovine chromaffin cells (BCCs) before and after cryopreserving with liquid nitrogen. Methods The APA microencapsulated BCCs were cryopreserved with dimethyl sulfoxide as cryopreservative agent by slow cooling and rapid rewarming for revivification. The change of cell function was observed by detecting the cell viability and secretion of catecholamine. Results As compared with the precryopreseving cells, the morphology and cell viability of the resuscitated APA microencapsulated BCCs showed no significant change. The catecholamine secretion volume of BCCs remained 80% of that by the precryopreserving cells. Conclusion It demonstrates that the resuscitated cryopreserved APA microencapsulated BCCs still remained good morphology, cell viability and secretion function of catecholamine.