Anti-inflammation effect of pyrrolidine dithiocarbamate on Pseudomonas aeruginosa induced pneumonia in rats / 中国药理学通报

ABSTRACT

Objective To investigate the effect of pyrrolidine dithiocarbamate(PDTC)on Pseudomonas aeruginosa induced pneumoneia of rats.Metheds A PA induced pneumonia model was established in Sprague-Dawley(SD)rats.Sixty minutes before PA exposure,PDTC and normal saline were injected into abdominal cavity separately.At 3,9,and 24 h after PA exposure,the rats were sacrificed,and the wet/dry ratio(W/D)of lung tissue was measured and histopathologic changes were observed.The number of in-filtrated polymorphonuclear(PMN)leukocytes was calculated.Immunohistochemical staining with activated NF-?B antibody was performed to detect the expression of NF-?B in lung tissues.The changes of IL-8 levels in bronchoalveolar lavage were identified by ELISA.Results Histology findings demonstrated that PA exposure induced obvious changes in lung structure,and edema and pronounced inflammatory cells infiltration were observed.Both symptoms and lesions of lung were lesser in the rats of PDTC group than those of the PA group.Compared with PDTC group,the activation of NF-?B and the expression of IL-8 were significantly up-regulated after PA challenge 3~24 h(P