Relationship between nonalcoholic fatty liver disease and levels of serum adiponectin and tumor necrosis factor-? in obese rats and pioglitazone intervention / 中国药理学通报

ABSTRACT

Aim To investigate the relationship between nonalcoholic fatty liver disease(NAFLD)and levels of serum adiponectin and tumor necrosis factor-?(TNF-?)in diet-induced obese rats and the effects of pioglitazone.Methods 45 SD male rats were randomly divided into three groupspioglitazone group(P),high-fat diet group(HF),normal control group(NC),15 rats in each group.Rats in NC group were fed with standard food and the others with high-fat diet.After 12 weeks,rats in P group were treated with pioglitazone 10mg/kg ig daily for 4 weeks.Accordingly,HF and NC groups were administrated with homologous solvent.Serum adiponectin,TNF-?,free fatty acid(FFA),and biochemical data were observed,and Lee's index,HOMA-IR and liver index were calculated.Pathologic histology and HE stain of rat livers were observed.Results Compared with HF group with NC group,body weight,liver index,levels of serum glucose,insulin,FFA,ALT,HOMA-IR and TNF-? increased significantly,and serum adiponectin decreased(P