A comparison of 2 kinds of hydroxyapatite/collagen composites as tissue engineering scaffold / 第二军医大学学报

ABSTRACT

Objective:To prepare 2 composites using different proportions of hydroxyapatite and collagen and to assess their structural and biological properties, so as to pave a way for preparing tissue engineering chest wall scaffold.Methods: Two kinds of hydroxyapatite/collagen composites were prepared according to the weight ratios of 11 and 12; collagen sponge served as control. Then the structures of the 2 composites and the collagen sponge were observed under SEM. In vivo study was conducted to assess the biocompatibility and biodegradation of the composites by gross inspection and histological examination. Results: The collagen sponge had a 3-D network structure with fluey collagen fibers and poor mechanical strength, and its structure was damaged within 2 weeks after implantation and was completely absorbed 4 weeks later. The hydroxyapatite and collagen were well mixed in the composite with a hydroxyapatite to collagen ratio of 12; the composite had homogeneous 3-D porous structure (size of the pore being 100-400 m) and showed good biocompatibility maintained its porous structure 4 weeks after implantation and was absorbed within 8 weeks. In composite with hydroxyapatite to collagen ratio of 11, the hydroxyapatite particles were separated from collagen fiber and conglomerated into masses, and the composite resulted in severe tissue reaction after implantation.Conclusion: When mixed with a reasonable proportion of hydroxyapatite, the collagen sponge has improved structure, biodegradable performance, and biocompatibility; the composite may be a novel scaffold for tissue engineering chest wall reconstruction.