Oxidative Damage of Mouse Liver and Lung Protein Induced by Multi-walled Carbon Nanotube Exposure / 医学研究杂志

ABSTRACT

Objective To study the protein oxidative damage and its possible mechanism caused by multi-walled nanotubes(MWCNTS)in mice.Methods Totally 20 Kun-ming mice were divided randomly into 4 testing groups(n=5 for each group),with 0.1,0.2 and 0.4mg/ml MWCNTS suspension injected groups and saline injected group as control group.After 5 days exposure,the protein carbonyl content was measured by using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage.Results The results showed that the protein carbonyl content in livers in 0.2 and 0.4mg/ml MWCNTS suspension injected groups were significantly higher than that in saline group(P