Construction of eukaryotic expression recombinant plasmid containing functional regeion of LIM mineralization protein-3 / 重庆医科大学学报

ABSTRACT

Objective:To construct an eukaryotic expression recombinant plasmid containing functional regeion of LIM mineral- ization protein-3.Methods:Functional regeion of LIM mineralization protein-3 was amplified by PCR with primers,then inserted into pEGFP-N1 to generate eukaryotic expression plasmid pEGFP-N1-LMP-3.The recombinant plasmid was identified by re- striction enzyme and sequencing.Results:The gene fragment encoding LMP-3 was amplified correctly by PCR.Enzyme diges- tion and identical sequencing showed that the recombinant eukaryotic expression plasmid was right constructed.Conclusion: Recombinant eukaryotic expression plasmid of LMP-3 has been successfully obtained,suggesting a foundation for the further studies on the bone formation.