Measurement of Alveolar Surfactant after Acrylsmide-induced Lung Injurids / 医学研究杂志

ABSTRACT

Objective To observe the change of composition of PL and content of protein in PS after anatoxin damnify lung.Methods The rats lung injury models were made by intratracheally instilling anatoxin(0.1mg/kg,0.4mg/kg).24 rats were divided into four groups:12hour group,2day group,3day group,5day group.Pr of each group were examined by electron microscope,content of PL,composition of PL and content of protein of each group wete determinde respectively.Results Rats lungs in experimental guoups were found that PS lost continuously,dropped in the pulmonary alveolies.12hour group was more apparent.Content of protein in PS was the highest in 12hour group,Content of PL in PS go add,12hour group was more apparent.Conclusion Morphologic change and alternation of quality and quantity of PS after anatoxin-induced pulmonary injures specifically reflect the activity of lung danmify.Measuring content of PL and PI is sign of simple and feasible method when lungs of the rats are injured early by bleomycin.