Comparing the Proliferation,Differentiation and Apoptosis of the Neural Stem Cells from Embryonic Brains of Different Stages / 医学研究杂志

ABSTRACT

Objective With the comparison of the proliferation,differentiation and apoptosis of neural stem cells from different embryonic stages,to obtain the biology of NSCs and the development mechanism of embryonic cortex underlining.Methods After the prime culture of NSCs from forebrain cortice of different embryonic stages(E14,E18 and E20),enzyme immunoassay for NSCs proliferation was carried out.After 7 days of differentiation induced by fetal bovine serum,they were detected by the neuronal marker MAP2 and the astrocyte marker GFAP with nuclei staining by Hoechst 33342 additionally.The ratios of MAP2+/Hoechst+ and GFAP+/Hoechst+ were calculated.Fluorescent activated cell sorting for apoptosis were used to detect the apoptosis of NSCs by Annexin V-FITC and propidium iodide.Results The absorbance of NSCs is 1.1771?0.0422(n=22) for E14,0.4127?0.0328(n=23) for E18,and 0.4127?0.0456(n=16) for E20(P