Effects of 2,3,4′,5-tetrahydroxystilbene-2-O-?-D glucoside on expression of ICAM-1、VCAM-1 and VEGF excited by ox-LDL in U937 cell / 中国药理学通报

ABSTRACT

Aim To observe the effects of 2,3,4′,5-tetrahydroxystilbene-2-0-?-D glucoside on expression of ICAM-1、VCAM-1 and VEGF excited by ox-LDL in U937 cell.Methods Differentiated U937 cells were cultured in vitro,excited by ox-LDL and intervened by different concentrations of TSG,then the level of SOD and MDA were determined.ICAM-1、VCAM-1 and VEGF protein were determined by Western blot and ICAM-1 mRNA and VCAM-1 mRNA were measured by reverse transcriptase-polymerase chain reaction(RT-PCR).Results In comparison with those of the control,the level of SOD remarkably decreased and the level of MDA remarkably increased in model group;the level of SOD increased markedly and the level of MDA decreased markedly in Simvastatin group and TSG 120 mg?L-1 group(P