ZO-1 may participate in regulatory effect of VASP on intestinal barrier function during hemorrhagic shock in rats / 第三军医大学学报

ABSTRACT

Objective To observe the relationship between zonula occludens protein subunit 1 (ZO-1) and the regulatory effect of vasodilator stimulated phosphoprotein (VASP) on the intestinal barrier function in rats of hemorrhagic shock (HS). Methods The intestinal barrier function was reflected by the hematoplasma D-lactic acid content,and the mucous membrane of small intestine from HS rats at different time after shock (1,2 and 4 h) were adopted to determine the expressions of VASP,phospho-VASP and ZO-1. cAMP,the agonist of VASP phosphorylation,was employed to observe the change of the above mentioned molecules and the intestinal barrier function following HS. Results (1) VASP expression of small intestine mucous membrane was decreased significantly after HS (P