Role of HO-1 in acute rejection after orthotopic liver transplantation in rats / 第三军医大学学报

ABSTRACT

Objective To investigate the effect of HO-1 on acute rejection after orthotopic liver transplantation (OLT) in rats. Methods Taking dark agouti (DA) rats as donors and Lewis rats as recipients, OLT was performed. Before OLT, the DA rats and Lewis rats were matched in pairs, and divided into control group, ZnPP group and CoPP group, the paired rats of which intraperitoneally received normal saline, 20 mg/kg ZnPP and 5 mg/kg CoPP respectively 24 h before OLT. Ten pairs of rats were in each group. On day 3 or 7 after OLT, recipient rats were killed, then blood sample and liver specimen were collected. Plasm levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), albumin (ALB) were measured; HE staining and MPO staining were performed; The expression of HO-1 was detected by the fluorence real time RT-PCR. Results On day 3 and 7 after OLT, AST, ALT and TBIL showed significant statistical significance among the three groups; ALB was highest in CoPP group, then in control group, lowest in ZnPP group. On day 3 after OLT, control group had acute rejection of degree one to degree two, ZnPP group had acute rejection of degree two; CoPP group mainly had acute rejection of degree one; On day 7 after OLT, control group mainly had acute rejection of degree two; ZnPP group had acute rejection of degree two to degree three; CoPP group had acute rejection of degree one to two. The expression of HO-1 was highest in CoPP group, then control group, and lowest in ZnPP group. Conclusion The expression of HO-1 in OLT with acute rejection in rats can palliate the acute rejection.