Inhibition of UVA-induced apoptosis by the polypeptide from Chlamys farreri via regulation of c-jun and COX-2 expression in HaCaT cells / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)2003.
Article
em Zh
| WPRIM
| ID: wpr-564555
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WPRO
ABSTRACT
Aim A UVA-induced apoptotic model of HaCaT cells was established to investigate the impact of UVA on c-jun/cyclooxygenase-2(COX-2)and explore related molecular mechanism of the polypeptide from Chlamys farreri(PCF)protecting HaCaT cells from UVA-induced apoptosis.Methods Cells were divided into five groups:control group,UVA model group,UVA+5.69 mmol?L-1 PCF group,UVA+2.84 mmol?L-1 PCF group,UVA+1.42 mmol?L-1 PCF group.Expression level of c-jun was assayed by Real-Time PCR and Western blot.RT-PCR and Western blot analysis were used to determine the mRNA and protein levels of COX-2.Using agarose gel electrophoresis,the effects of PCF and COX-2 inhibitor celecoxib on UVA-induced apoptosis were also investigated.Results PCF and celecoxib had inhibitory effect on 8 J?cm-2 UVA-induced apoptosis of HaCaT cells.COX-2 mRNA and protein levels increased after UVA radiation and the discrepancy was significant compared with control group(P
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Idioma:
Zh
Revista:
Chinese Pharmacological Bulletin
Ano de publicação:
2003
Tipo de documento:
Article