Isolation and identification of tumor-like stem cells from human nasopharyngeal carcinoma cell line / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)2001.
Artigo
em Chinês
| WPRIM
| ID: wpr-564611
ABSTRACT
Objective To investigate the expression of CD133 in human nasopharyngeal carcinoma cell line SUNE,and to detect proliferative and differential ability of CD133+ tumor cells in vitro,and to identify tumor-like stem cells in SUNE.Methods Immunocytochemical staining method and flow cytometry were used to detect the expression of putative tumor-initiated cell marker CD133 in nasopharyngeal carcinoma cell line SUNE,and the isolation technique with immunomagnetic beads was applied to purify CD133+ cells.The proliferative ability of CD133+ tumor cells in vitro was estimated by MTT assay,and the proliferative ability of CD133+,CD133-and control SUNE cells were statistically compared.Finally,the immunocytochemical staining method and flow cytometry were used to detect differential ability of CD133+ tumor cells in vitro.Results CD133 was positively expressed only in 0.35% of the cells in nasopharyngeal carcinoma cell line SUNE.In the serum-free medium RPM I1640,the UV optical density of the CD133+ tumor cells enriched with immunomagnetic beads was 0.317?0.007,0.370?0.002 and 0.558?0.004 at 3,5 and 7 days,respectively.Compared with CD133-cells and control SUNE cells,CD133+ cells showed increased proliferative capacity(P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Medical Journal of Chinese People's Liberation Army
Ano de publicação:
2001
Tipo de documento:
Artigo
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