Differential Analysis of Mutation of Fibroblast Cells Before and After Cadmium Treatment / 医学研究杂志

ABSTRACT

Objective Because in the environment there are many compounds to cause variation of cells, the study for high sensitivity’s method in which the variation of cells by environment can be tested is necessary. For example, the reports of detailed study for the application of cadmium on the human body cell’s lethal toxicity and the variation of cells are very few. This research is about a high sensitivity method of heavy metal cadmium’s variation active processed by cadmium in which the variation of cells can be tested by ultraviolet ray. Methods The study used cultured cells of human being and derivation. The assay of mutagenicity OuaR (Ouabain resistance) variation used picking law and Differential dot-blot of cancer gene K-ras variation the hybridization examination method. In order to test the induction’s frequency of cell variation, MTT (methyl thiazolyl tetrazoliun) and survival efficiency of formation were tested. The clarifing of mutagenicity mechanism used Western blot and the siRNA method to analysis the molecular of heat shock protein. Results Cadmium clarifiing mutagenicity variation detection had the same degree with the method of ultraviolet ray. The DNA was injury corresponding to the HSP27 protein cell’s change.The cell variation frequency also presented the high value. Conclusion Human fibroblasts in the role of the heavy metal cadmium showed that HSP27 protein expression in high-UVr-1 cells RSa has lowor-frequency variations than OuaR cells. K-ras dot blot method proved that RSa cells variation is higher than the cells UVr-1 mutation. In siHSP27 cells, we observed OuaR high variability of expression. Cadmium on DNA damage showns UVr-1 fibroblast cell is lower than RSa fibroblasts cell.