Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Journal of Veterinary Science
;
: 119-122, 2016.
Artigo
em Inglês
| WPRIM
| ID: wpr-56503
ABSTRACT
The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Brucella abortus
/
Brucelose
/
Ensaio de Imunoadsorção Enzimática
/
Doenças dos Bovinos
/
Clonagem Molecular
/
Escherichia coli
/
Malato Desidrogenase
/
Antígenos de Bactérias
Tipo de estudo:
Estudo de rastreamento
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2016
Tipo de documento:
Artigo
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