Your browser doesn't support javascript.
loading
In vitro cultivation and identification of epithelial cells of keratocystic odontogenic tumor / 北京大学学报(医学版)
Journal of Peking University(Health Sciences) ; (6)2004.
Artigo em Chinês | WPRIM | ID: wpr-565045
ABSTRACT

Objective:

To establish a method of in vitro cultivation of epithelial cells of keratocystic odontogenic tumors(KCOT).

Methods:

Tissue block and enzyme digestion techniques were used for primary cultivation of KCOT cells.The cells were grown in keratinocyte-serum free medium(K-SFM).The biological characteristics of the cultivated keratinocytes were identified by phase microscopic observation and by immunohistochemistry of cytokeratin,vimentin,cytokeratin10 and 14.

Results:

KCOT keratinocytes could survive for 30-50 days in K-SFM after passing 2-4 generations.Cells were polygon and showed typical slabstone-like appearance.Immunostaining showed positive staining for cytokeratin antibody,and negative for vimentin.

Conclusion:

KCOT epithelial cells could be serially cultured in vitro in K-SFM by techniques suggested in this study.

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Chinês Revista: Journal of Peking University(Health Sciences) Ano de publicação: 2004 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Chinês Revista: Journal of Peking University(Health Sciences) Ano de publicação: 2004 Tipo de documento: Artigo