Regulatory mechanism of transforming growth factor-beta 1 on mechanical properties of tissue engineered heart valve / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1982.
Artigo
em Chinês
| WPRIM
| ID: wpr-565056
ABSTRACT
Objective:
To observe whether transforming growth factor-beta 1 (TGF-?1) can improve the mechanical properties of tissue engineered heart valve (TEHV) prepared by TGF-?1 and myofibroblasts.Methods:
Myofibroblasts were isolated,cultured,identified,and seeded onto the decellularized aortic valve leaflet.In the experimental group,TEHV were cultured with DMEM containing 10 ng/ml TGF-?1 for 14 days.In the control group,TEHV were cultured with DMEM only for 14 days.The contents of lysyl oxidase (LOX) and hydroxyproline,the expression of LOX and COLL-1 mRNA,and the mechanical properties of the TEHVs were analyzed in each group.Results:
The contents of LOX and hydroxyproline,expression of LOX and COLL-1 mRNA,Max-load,and Max-stress were significantly higher in the experimental group than those in the control group (all P
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo prognóstico
Idioma:
Chinês
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
1982
Tipo de documento:
Artigo
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